Insertion junction: LMJ.RY0402.102730_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.102730
Chromosome:chromosome 6
Location:6233998
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g290800 RMG1 RNA cap guanine-N2 methyltransferase; (1 of 1) 2.3.1.221 - Noranthrone synthase / Polyketide synthase A 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAAGGTGGTCTGTGTATAGCAAAGGAAC
Internal bar code:CGGGGATAGGGAACAGGATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:738
LEAP-Seq percent confirming:98.7723
LEAP-Seq n confirming:1609
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGGCATCACCCTGTATTG
Suggested primer 2:GTTCTACTCGAGGCAGGACG