| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102773 |
| Chromosome: | chromosome 4 |
| Location: | 2100185 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219300 | (1 of 1) PF02958 - Ecdysteroid kinase (EcKinase); Putative choline kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTTGCTGGCTTGTTGATCCAGGCTGAT |
| Internal bar code: | CAGTGGAGCCCTTTTTTTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 99.6759 |
| LEAP-Seq n confirming: | 5843 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGGGTCGCTTATGGTCA |
| Suggested primer 2: | GACTACCTGGCCCACTACCA |