Insertion junction: LMJ.RY0402.102794_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.102794
Chromosome:chromosome 3
Location:2591663
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g160600 (1 of 1) IPR000104//IPR000679//IPR013088 - Antifreeze protein, type I // Zinc finger, GATA-type // Zinc finger, NHR/GATA-type 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCTTCGCGACAAAATTGAGACGAAGGGA
Internal bar code:CCGGCCAAACATCGTCACTCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:72
LEAP-Seq percent confirming:84.3373
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TATACGGAGCACTTTGCACG
Suggested primer 2:TTGCTCCGCCACCTACTACT