| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.102839 |
| Chromosome: | chromosome 6 |
| Location: | 1884162 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263200 | FAP325 | Flagellar Associated Protein 325 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTAGCAGCCAAGTTTGCACCTGGGCCCA |
| Internal bar code: | GTGAAGGGGCGGCTAGATGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.2288 |
| LEAP-Seq n confirming: | 772 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGTCGTAACCTGCCATC |
| Suggested primer 2: | GATGGATGTTCTTCTGCGGT |