| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102881 |
| Chromosome: | chromosome 14 |
| Location: | 3691909 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631750 | BLZ12 | bZIP transcription factor; (1 of 10) PF00170 - bZIP transcription factor (bZIP_1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAATTAATAACAGCTGTCACTTGATACA |
| Internal bar code: | CGTTGGCGTCGTGTAGTCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 99.3056 |
| LEAP-Seq n confirming: | 429 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTACCATGGAGCGAAAT |
| Suggested primer 2: | AAGTAGCAAGCGCGAGAGAG |