| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102886 |
| Chromosome: | chromosome 10 |
| Location: | 5763992 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461150 | serine/threonine protein kinase; (1 of 38) IPR000719//IPR002290//IPR011009 - Protein kinase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCCATGTGTGCCCATGTAGTGAAAAGG |
| Internal bar code: | CTCCCTACCCCACGTTCTAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 962 |
| LEAP-Seq percent confirming: | 99.6241 |
| LEAP-Seq n confirming: | 1325 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCCGCTTTAATAACCAA |
| Suggested primer 2: | AAATTGAAAGGACCCCCAAC |