Insertion junction: LMJ.RY0402.102899_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.102899
Chromosome:chromosome 9
Location:6588453
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g408350 DeSI-5,DESI5 DeSI-type SUMO protease; (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTCCTGAACCCCATCGGCTGGGGCAGCC
Internal bar code:TTGACGAATCGCGGAACTGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:640
LEAP-Seq percent confirming:99.3151
LEAP-Seq n confirming:1305
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGGGCGTGTCTTACTTGG
Suggested primer 2:GGTTGGTATACATACGGCGG