Insertion junction: LMJ.RY0402.102901_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.102901
Chromosome:chromosome 3
Location:7601314
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g204601 CPK3,FAP278 Flagellar Associated Protein 278; (1 of 1) K00856 - adenosine kinase (E2.7.1.20, ADK) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGCAGCGCGCGAAGTGGCGCGCAGGTA
Internal bar code:CGAACTCAGCGACCGGTGCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:671
LEAP-Seq percent confirming:99.3469
LEAP-Seq n confirming:1217
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGATCAGAGTTCGCAGGTTG
Suggested primer 2:AGCCATTCAACAGTTCTGGG