| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.102901 |
| Chromosome: | chromosome_3 |
| Location: | 7601314 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g204601 | FAP278,CPK3 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGGTGCAGCGCGCGAAGTGGCGCGCAGGTA |
| Internal bar code: | CGAACTCAGCGACCGGTGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 99.3469 |
| LEAP-Seq n confirming: | 1217 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCAGAGTTCGCAGGTTG |
| Suggested primer 2: | AGCCATTCAACAGTTCTGGG |