| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102902 |
| Chromosome: | chromosome 3 |
| Location: | 7202663 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207900 | CYCA1 | (1 of 3) K06627 - cyclin A (CCNA); A-type cyclin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCACCCGTGCGCTGTCGGCTACACCCCA |
| Internal bar code: | TAACCGATGTGTATATCAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 99.7546 |
| LEAP-Seq n confirming: | 813 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTCCTTTGGGGTCCTTC |
| Suggested primer 2: | CACGGTCAGCTCGTACTTGA |