| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102906 |
| Chromosome: | chromosome 3 |
| Location: | 8153879 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200550 | HYD3 | Iron hydrogenase/prelamin A binding protein; (1 of 1) PTHR11615:SF103 - CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NARFL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCTGTGGCCGTACGCCCGTACCCCGGC |
| Internal bar code: | ACTTTGGAGTTAGTGGCGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 99.5991 |
| LEAP-Seq n confirming: | 4969 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCCCAATTTTCAATACC |
| Suggested primer 2: | GACACGGATAATGCACGTTG |