| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102939 |
| Chromosome: | chromosome 2 |
| Location: | 4961583 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103900 | MAPKKK11 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Mitogen-Activated Protein Kinase Kinase Kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAATGCTCGTGAGGCAGTCTGGATCCAG |
| Internal bar code: | ACTCGGGGGATCAACCCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 92.8775 |
| LEAP-Seq n confirming: | 652 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCGGTACCGGTTATGCT |
| Suggested primer 2: | CGGCCTGTACATACGTCCTT |