| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102956 |
| Chromosome: | chromosome 14 |
| Location: | 1460776 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618000 | EFH9 | EF-hand Calcium binding protein; (1 of 5) PTHR23050:SF90 - CENTRIN-3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGATCGGAGGCCTCACTCACAACTCAC |
| Internal bar code: | AATTCGGTGCCTAGCTAGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1033 |
| LEAP-Seq percent confirming: | 99.4759 |
| LEAP-Seq n confirming: | 3986 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCAAATTAGCACCAGCAC |
| Suggested primer 2: | AGCGCTTGCCAAGTTAGGTA |