| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.103073 |
| Chromosome: | chromosome_9 |
| Location: | 6769632 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g409325 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTTGCCAACGTCATCGCCGGCTTCACAG |
| Internal bar code: | GCGGTTATGCAGGGAGGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 99.4209 |
| LEAP-Seq n confirming: | 2747 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAAAACGTAAGTGGGAAA |
| Suggested primer 2: | GATGAAGCCTCTCCTGCTTG |