| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103074 |
| Chromosome: | chromosome 1 |
| Location: | 4027082 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026150 | (1 of 3) 3.4.24.59 - Mitochondrial intermediate peptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTGCTTGAGTTGTGGCAGTGCGGCCTTT |
| Internal bar code: | GTGGTAAGCGCCGGTTGCTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 98.1043 |
| LEAP-Seq n confirming: | 3312 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGATGGGACAGTTGTAAG |
| Suggested primer 2: | ACACACACACACACACACCG |