| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103101 |
| Chromosome: | chromosome 1 |
| Location: | 6114188 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043650 | LRRC56,MOT37,ODA8,DLU2 | Outer Arm Dynein Assembly protein 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGCACGACGCCATACGCAAGAGAAGCC |
| Internal bar code: | GGGGTCTGAATCATCCGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 98.1642 |
| LEAP-Seq n confirming: | 3957 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCAAAATTTGGCAAGGG |
| Suggested primer 2: | GTCTGGGTGCTCCTCTTCAG |