| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103173 |
| Chromosome: | chromosome 4 |
| Location: | 490858 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217930 | (1 of 1) PF06911 - Senescence-associated protein (Senescence) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGTACCGGTGCTCATGATTTGTAACTA |
| Internal bar code: | GGTACATTGCTCTCAGAACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 99.7975 |
| LEAP-Seq n confirming: | 5913 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCGACAGAGCTTGGCAGT |
| Suggested primer 2: | GGTGGTGTGGCTACAGGAGT |