Insertion junction: LMJ.RY0402.103173_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.103173
Chromosome:chromosome 4
Location:490871
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTCACCTCTAGCCTGTAACCAGACGGAA
Internal bar code:GCGCCAGCACCCCTATCCAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:316
LEAP-Seq percent confirming:72.2045
LEAP-Seq n confirming:1808
LEAP-Seq n nonconfirming:696
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AATCGACAGAGCTTGGCAGT
Suggested primer 2:GGTGGTGTGGCTACAGGAGT