Insertion junction: LMJ.RY0402.103178_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.103178
Chromosome:chromosome 2
Location:109273
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g074000 POC18 Proteome of centriole protein 18; (1 of 1) PTHR19853//PTHR19853:SF1 - WD REPEAT CONTAINING PROTEIN 3 WDR3 // TBC1 DOMAIN FAMILY MEMBER 31 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCTGCAGCGGCAGCAGGCGCCGGAGGAG
Internal bar code:GCAAAATACAGGCGGTGACGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:498
LEAP-Seq percent confirming:99.2173
LEAP-Seq n confirming:1648
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CAACTTCACACTGTTGCGCT
Suggested primer 2:TACGAACACCCACCAGATCA