Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.103178 |
Chromosome: | chromosome_2 |
Location: | 109273 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g074000 | POC18 | Centriole proteome protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGCCTGCAGCGGCAGCAGGCGCCGGAGGAG |
Internal bar code: | GCAAAATACAGGCGGTGACGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 498 |
LEAP-Seq percent confirming: | 99.2173 |
LEAP-Seq n confirming: | 1648 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAACTTCACACTGTTGCGCT |
Suggested primer 2: | TACGAACACCCACCAGATCA |