Insertion junction: LMJ.RY0402.103186_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.103186
Chromosome:chromosome 10
Location:3297438
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g443400 (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACACCAGGACCGGCCCCGGCACCGTGACG
Internal bar code:GTCTATCGTATGGGGGATGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:575
LEAP-Seq percent confirming:98.3913
LEAP-Seq n confirming:2997
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCACAAGCTCCAGTACAT
Suggested primer 2:AAACTCAAGTCAACCCGGTG