| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103209 |
| Chromosome: | chromosome 1 |
| Location: | 1694055 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008950 | (1 of 1) IPR024930 - Skp domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCACTTCTCGCTGTCGCCTATTTACATG |
| Internal bar code: | GCAGGTCCTATCTCAGCAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 103 |
| LEAP-Seq percent confirming: | 11.3125 |
| LEAP-Seq n confirming: | 181 |
| LEAP-Seq n nonconfirming: | 1419 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTCCGTGCATTCCGTCT |
| Suggested primer 2: | AAAGGTTGTGCAGTTTTGGG |