Insertion junction: LMJ.RY0402.103250_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.103250
Chromosome:chromosome_13
Location:4615089
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g604050 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TGTATGACGTGACATGGCAGACACCGGCAC
Internal bar code:GTTTCTCAGAGATTTTGTGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:301
LEAP-Seq percent confirming:99.373
LEAP-Seq n confirming:317
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGTTACACACGCATACAC
Suggested primer 2:AAACAACAAGGGGTGTCAGC