| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103261 |
| Chromosome: | chromosome 7 |
| Location: | 3912193 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339104 | (1 of 2) 3.6.3.19 - Maltose-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACCCTCGCTGCATGTACACACTCACTG |
| Internal bar code: | GACGCTCGCATCGACTGTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 99.076 |
| LEAP-Seq n confirming: | 4182 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGTCCCACCAGGAGTGT |
| Suggested primer 2: | CTCAATGCCAATGGACACAC |