Insertion junction: LMJ.RY0402.103263_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.103263
Chromosome:chromosome_14
Location:866201
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre14.g613400 POC16 Centriole proteome protein sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TCCCCTCTCTCCTCCCTCTCCCCTCCCTCT
Internal bar code:GAGTGTTGTGGGTTGCAGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:572
LEAP-Seq percent confirming:88.7417
LEAP-Seq n confirming:134
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCTCTTTAACACACGCCT
Suggested primer 2:TAGGGAGTTGGACCGAGTTG