| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103277 |
| Chromosome: | chromosome 8 |
| Location: | 4902753 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385000 | (1 of 38) IPR000719//IPR002290//IPR011009 - Protein kinase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGTAGAGCCAAAGCCCCACTCAGGCAC |
| Internal bar code: | ATGAGTACCGATGTAGGTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 351 |
| LEAP-Seq percent confirming: | 99.7918 |
| LEAP-Seq n confirming: | 1438 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGTGGAGCTGAAGGAGC |
| Suggested primer 2: | CACATACCCCTTGTGCCTCT |