Insertion junction: LMJ.RY0402.103298_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.103298
Chromosome:chromosome 7
Location:915573
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g318950 (1 of 1) IPR000008//IPR000104//IPR003072 - C2 domain // Antifreeze protein, type I // Orphan nuclear receptor, NOR1 type 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGTGCGCGAACCTGGCGCTGAGGCAGA
Internal bar code:CAGTGAGGCCGTTCATCGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:558
LEAP-Seq percent confirming:98.5992
LEAP-Seq n confirming:1267
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGACTGACTACGGGTGGC
Suggested primer 2:TAGCTGCCATCGCTAATGTG