| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103299 |
| Chromosome: | chromosome 3 |
| Location: | 2515658 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159950 | (1 of 1) K14788 - ribosome biogenesis protein ENP2 (NOL10, ENP2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTCGTTCGTGCCGCGCAGCGGTGGCG |
| Internal bar code: | GCCTGGACACACCTTTCACCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 50.9951 |
| LEAP-Seq n confirming: | 7200 |
| LEAP-Seq n nonconfirming: | 6919 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAGCTTAATTGCTTCCA |
| Suggested primer 2: | ACCACTACCACCACCACCAT |