| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103307 |
| Chromosome: | chromosome 6 |
| Location: | 5084541 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280650 | CGL59,Y3IP1 | Chloroplast Ycf3 interacting protein involved in Photosystem I biogensis | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCGCAGCGCGCGCGGGAGTTTTGGGCT |
| Internal bar code: | GGAGGATTACCGTGGCGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 99.1604 |
| LEAP-Seq n confirming: | 1181 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGAGCAGCTATGAGGAC |
| Suggested primer 2: | AAGAAGGAGGAGGAGGAACG |