| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.103313 |
| Chromosome: | chromosome 12 |
| Location: | 2747246 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503300 | (1 of 1) 4.1.1.21 - Phosphoribosylaminoimidazole carboxylase / AIR carboxylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCTCGCGCGCACAAACCGGCACGCCA |
| Internal bar code: | GGGAGAGAGGCTGGGGGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 99.4203 |
| LEAP-Seq n confirming: | 686 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGCGTGCAGAACCATAG |
| Suggested primer 2: | TCTCCTTTCTGAATCCCCCT |