| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103322 |
| Chromosome: | chromosome 12 |
| Location: | 5254207 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528350 | (1 of 1) IPR000408//IPR001810//IPR009091 - Regulator of chromosome condensation, RCC1 // F-box domain // Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCGGCTAACACATCTTGCGTTGCTTGC |
| Internal bar code: | TACGGTGGCCCCCAACCTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 280 |
| LEAP-Seq percent confirming: | 99.4262 |
| LEAP-Seq n confirming: | 2426 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATTGCTGTTTTGTGGGTG |
| Suggested primer 2: | CATGGTCTCCTCTTCCGTGT |