| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103336 |
| Chromosome: | chromosome 12 |
| Location: | 4647555 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523050 | CCT11,GEX204 | (1 of 1) 2.7.1.150 - 1-phosphatidylinositol-3-phosphate 5-kinase / Type III PIP kinase; Putative T-complex protein 1 component | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCAAATCCCCGCGCCCAGCCAGCTGCC |
| Internal bar code: | AAGACACATTCAAAGCTCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 99.4969 |
| LEAP-Seq n confirming: | 2571 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGAGTGCTGACCAACTG |
| Suggested primer 2: | CTTGTGCTCACCTCCTCCTC |