| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103343 |
| Chromosome: | chromosome 16 |
| Location: | 1204441 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650800 | TIM13 | Mitochondrial inner membrane translocase subunit; (1 of 1) K17781 - mitochondrial import inner membrane translocase subunit TIM13 (TIM13) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGACCGCTGATCCACGGTGACGGGTAC |
| Internal bar code: | CTGATTCTTCTAACCGGTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.3869 |
| LEAP-Seq n confirming: | 3080 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATTACTCGCAAGCGTTGA |
| Suggested primer 2: | TGCACCGACGTTCACATATT |