| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.103346 |
| Chromosome: | chromosome_2 |
| Location: | 571928 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g077600 | FAP51 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTGCGTGCGGGCGCATGCGTGCTTGGCAA |
| Internal bar code: | GACGTCGGCCATAGAAGCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 83.6434 |
| LEAP-Seq n confirming: | 34078 |
| LEAP-Seq n nonconfirming: | 6664 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCTTGTCTCCGCTTTCT |
| Suggested primer 2: | CGGGCTTCGTTATGTTGTTT |