| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.103349 |
| Chromosome: | chromosome 10 |
| Location: | 2366661 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCCAGGAGGGCAGGATGTCAGGAGCAG |
| Internal bar code: | GGGCTACGATGCACCTACGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 97.1072 |
| LEAP-Seq n confirming: | 1477 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACCTCGCTGTGGCTATC |
| Suggested primer 2: | TGAGTTTGTGTTGCGAAAGC |