| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103364 |
| Chromosome: | chromosome 1 |
| Location: | 7527931 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054250 | CYP4,CYP743A1 | Cytochrome P450, CYP5 superfamily, CYP5A family; (1 of 19) 1.14.14.1 - Unspecific monooxygenase / Xenobiotic monooxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAATCTGGCAGTCCCACCCACATGCGCG |
| Internal bar code: | GCGATAACCTGCGTAGCGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 265 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTGGCTAGGACTCACTG |
| Suggested primer 2: | CGGTGCATAGCTCAATCAGA |