| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103395 |
| Chromosome: | chromosome 9 |
| Location: | 6801308 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409600 | FAP411 | Flagellar Associated Protein 411 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCAAGTGTTCTGTACGGTGGGTGGGGC |
| Internal bar code: | CTCCAAAGGTTGCAGACCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 99.7486 |
| LEAP-Seq n confirming: | 3968 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTTGGAAGAAGCATGGG |
| Suggested primer 2: | CTGCATCCTCCACAGCACTA |