| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103439 |
| Chromosome: | chromosome 11 |
| Location: | 1507801 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467745 | GCP3 | (1 of 1) K16570 - gamma-tubulin complex component 3 (TUBGCP3, GCP3); Gamma tubulin interacting protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCTCCGCGCTACTTAGGCCCGTGCAGC |
| Internal bar code: | ACCACTCTATCCCACGCGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 98.4733 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGAGCAGGTTGTCGAGG |
| Suggested primer 2: | TTCTTACATTGAATCGCCCC |