| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103445 |
| Chromosome: | chromosome 10 |
| Location: | 2858861 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439750 | (1 of 6) PTHR24114:SF2 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGGGGCGTGTGCTGGTGGGCCGGAGGGT |
| Internal bar code: | TTAGGCGTGAATGGCTCCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 99.0909 |
| LEAP-Seq n confirming: | 218 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAGGTAAGGAGTGAGAC |
| Suggested primer 2: | AATGCCGTACGGTACTGACC |