Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.103447 |
Chromosome: | chromosome_8 |
Location: | 1135406 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g362650 | IFT140 | Intraflagellar transport protein 140 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGTTGCCCGCCCATGCCACCTGAAGGTTTG |
Internal bar code: | CGGCACAGGGACGTCTCTATCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1055 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 78 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAATGACCAGGGCTACAGGT |
Suggested primer 2: | GTTGGGCTGGTTGAACTTGT |