| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103457 |
| Chromosome: | chromosome 7 |
| Location: | 4351748 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341251 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAAAGTTGGGACACATAGAGAACCGGAA |
| Internal bar code: | CCGTCACTCTTGTCATTTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 98.8668 |
| LEAP-Seq n confirming: | 10644 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAATCAGGAACAGTGGGAA |
| Suggested primer 2: | CCCCATACATGCGCTATCTT |