Insertion junction: LMJ.RY0402.103457_5

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.103457
Chromosome:chromosome 7
Location:4351748
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g341251 (1 of 66) 2.7.12.1 - Dual-specificity kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAAAGTTGGGACACATAGAGAACCGGAA
Internal bar code:CCGTCCCTCTTGTCATTTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:366
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGAATCAGGAACAGTGGGAA
Suggested primer 2:CCCCATACATGCGCTATCTT