| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103463 |
| Chromosome: | chromosome 13 |
| Location: | 1127750 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569500 | (1 of 3) PTHR10696 - GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED; Similar to conserved hypothetical protein in bacteria | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGCATGCATGCCGTGTGGGGGGCAGTG |
| Internal bar code: | GTAGTTGGGAGTTGCGCTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAAACCCAAGTACCAAAT |
| Suggested primer 2: | GTCGCTGTAGTTGACGGACA |