| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.103466 |
| Chromosome: | chromosome 3 |
| Location: | 8922561 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g208497 | (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGAAATTTACCAGTCATCTAATCGTCAC |
| Internal bar code: | GTGCACTACGTAGATCGGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 97.5359 |
| LEAP-Seq n confirming: | 475 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGACGACCTTGCTGTCAC |
| Suggested primer 2: | CACACTGTGCTGAGAGCCAT |