| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103501 |
| Chromosome: | chromosome 8 |
| Location: | 4540376 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382700 | UBQ3 | (1 of 4) K08770 - ubiquitin C (UBC); Bi-ubiquitin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTGGCGCCGCGGCAGCCGGGCAGCAGC |
| Internal bar code: | AGGTATTTTGGGATTTGCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 99.7065 |
| LEAP-Seq n confirming: | 1019 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACGCGTACGACTCATGC |
| Suggested primer 2: | ATGAGATGCAGGGTGGACTC |