| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.103519 |
| Chromosome: | chromosome 16 |
| Location: | 1663628 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654600 | PHC56 | (1 of 71) PF12499 - Pherophorin (DUF3707); Pherophorin-chlamydomonas homolog 56 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGTACGTGCACGCTAAACGCCTGCCTT |
| Internal bar code: | TGTGGCGCAAGGCGTCCAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 98.1243 |
| LEAP-Seq n confirming: | 3505 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCCACGCTGCTGTACTCG |
| Suggested primer 2: | CTGGTCATGCCGGTAAAGTT |