| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.103528 |
| Chromosome: | chromosome 1 |
| Location: | 802529 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004124 | (1 of 3) 3.2.1.8 - Endo-1,4-beta-xylanase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGCGTGCCTGATAAAATGTACAACTAA |
| Internal bar code: | TGCCGGCTTCCTCCGCCTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 99.2047 |
| LEAP-Seq n confirming: | 6362 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACATGAGCCCGTGATAC |
| Suggested primer 2: | TGTTGCTTTGAATGGCAGAG |