Insertion junction: LMJ.RY0402.103530_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.103530
Chromosome:chromosome 4
Location:492789
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACTTTCGCCCACCTTGGCCCCACCTTC
Internal bar code:GGGGGTTAAACGTAGCGGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:447
LEAP-Seq percent confirming:82.716
LEAP-Seq n confirming:134
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ATAATCAGGGTCCACCACCA
Suggested primer 2:CCGTGTGTGTGTGTGTGTGT