Insertion junction: LMJ.RY0402.103531_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.103531
Chromosome:chromosome 17
Location:2690338
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g717850 PHC8 (1 of 1) IPR003882//IPR024616//IPR027417 - Pistil-specific extensin-like protein // Pherophorin // P-loop containing nucleoside triphosphate hydrolase; Pherophorin-chlamydomonas homolog 8 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGACCCCCCTGTCCATGTTCTGCCCAAA
Internal bar code:CGGGGTTCCGCGGTCACCGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:529
LEAP-Seq percent confirming:99.6657
LEAP-Seq n confirming:4472
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTACAGTCTTTCTGCGGC
Suggested primer 2:ATGGAACCACACAAAGCCTC