| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.103539 |
| Chromosome: | chromosome 5 |
| Location: | 1976516 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241640 | (1 of 1) IPR000253//IPR000859//IPR014710 - Forkhead-associated (FHA) domain // CUB domain // RmlC-like jelly roll fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACTGGTGCAAGGCTGCTGTAGCGGCTC |
| Internal bar code: | TTTGTGATATCCCAAGCCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 96.1589 |
| LEAP-Seq n confirming: | 5833 |
| LEAP-Seq n nonconfirming: | 233 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCAAATTGAAGCAGCAG |
| Suggested primer 2: | CCTGGCAAGGGGTACAGTAA |