Insertion junction: LMJ.RY0402.103557_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.103557
Chromosome:chromosome 4
Location:158116
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g216902 ODA16,DAW1,WDR69 (1 of 1) PTHR22847//PTHR22847:SF464 - WD40 REPEAT PROTEIN // DYNEIN ASSEMBLY FACTOR WITH WDR REPEAT DOMAINS 1; Outer Arm Dynein-IFT adaptor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCGTCTTCGCCACCACCCCCAACCTG
Internal bar code:AGGGGTACAGCTAGGGCTGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:177
LEAP-Seq percent confirming:99.2701
LEAP-Seq n confirming:136
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCACGAAGAAACCAGGAACC
Suggested primer 2:TGCTGTGTCTTCGTTTGGAC