Insertion junction: LMJ.RY0402.103565_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.103565
Chromosome:chromosome 13
Location:822856
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g567200 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAACAGTGCAGCTTCGGGGAACCCAGCA
Internal bar code:AATGAGGCTGCACGTTATTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:134
LEAP-Seq percent confirming:6.89906
LEAP-Seq n confirming:190
LEAP-Seq n nonconfirming:2564
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAAAGACCCGGTAGGTTT
Suggested primer 2:CAGGAGAAGCTGGTCTACGG